We narrowed to 43,146 results for: cha

1. iRFP713-FRB-Mss4-Kina

   Plasmid

   #202744

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. gRNA_tdtomato_reporter_1_SA-2x

   Plasmid

   #79369

   Purpose

   Assays activity of transcriptional activators fused to SA Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MXS_PGK::NeoR-bGHpA

   Plasmid

   #62438

   Purpose

   MXS_chaining vector with PGK::NeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Neomycin with PGK Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Psy074

   Plasmid

   #99504

   Purpose

   Bacterial expression of LIPS M1+TE

   Depositor

   Jay Keasling

   Article

   Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

   Insert

   LIPS M1+TE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Sc NybbR eRF3∆165N (WT)

   Plasmid

   #175419

   Purpose

   Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV and ybbR

   Expression

   Bacterial

   Mutation

   ∆165N

   Promoter

   T7

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILGFP3A2 (2)

   Plasmid

   #185852

   Purpose

   Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. POM42-mCitrine

   Plasmid

   #69854

   Purpose

   N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CFP_Rem_pcDNA4_HisMaxC

   Plasmid

   #41651

   Depositor

   Henry Colecraft

   Article

   Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

   Insert

   Rem, GTP binding proteins (RGK) (Rem1 Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NET39 mRFP-C1 (1219)

   Plasmid

   #62042

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET39 (PPAPDC3 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS416 Gal-RNQ1 153-405-YFP

    Plasmid

    #18689

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 153-405
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Prion domain of Rnq1

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pMM2_12

    Plasmid

    #183061

    Purpose

    Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMM2_10

    Plasmid

    #183059

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM2_9

    Plasmid

    #183058

    Purpose

    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM2_11

    Plasmid

    #183060

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HAA1 and Synthetic promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SB53

    Plasmid

    #21488

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1-65 deleted

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

17. S3_002_026_096

    Plasmid

    #134403

    Purpose

    pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    None and none

    Expression

    Yeast

    Mutation

    None and none

    Promoter

    pGPM1 and pRPL18B

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEos2-Talin-IBS2-7

    Plasmid

    #57424

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin IBS2 (Tln1 Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Talin Integrin Binding Site

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

    Plasmid

    #164917

    Purpose

    Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(PGK1)-citrine and P(HIS4)-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 (yeast) and HIS4 (yeast)

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tdEos-Vinculin-14

    Plasmid

    #57691

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

21. JBEI-6823

    Plasmid

    #84986

    Purpose

    For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-PMKco-Mkco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. JBEI-6833

    Plasmid

    #84983

    Purpose

    Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    NudB-PMD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. POM43-mCitrine

    Plasmid

    #69855

    Purpose

    N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pK3FS-PYK1

    Plasmid

    #85777

    Purpose

    N-ICE plasmid pK3FS-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTJK517

    Plasmid

    #121474

    Purpose

    CEN TRP1 CNA1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNA1
    

    Use

    Yeast cen vector

    Tags

    Expression

    Mutation

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDM3193

    Plasmid

    #113390

    Purpose

    Alpha factor inducible Ty3 with his3AI

    Depositor

    Suzanne Sandmeyer

    Article

    Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

    Insert

    Ty3-his3AI
    

    Use

    Tags

    his3AI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. S3_004_026_MV1

    Plasmid

    #134416

    Purpose

    pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gRNA_tdtomato_reporter_1_ST1_2x_binding

    Plasmid

    #79368

    Purpose

    Assays activity of transcriptional activators fused to ST1 Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JBEI-6822

    Plasmid

    #84985

    Purpose

    For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-MKco-PMKco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-oChD-citrine

    Plasmid

    #50976

    Purpose

    AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    oChD-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChD gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEMT-NRTN

    Plasmid

    #119896

    Purpose

    Mouse NRTN In situ hybridization probe

    Depositor

    Wenqin Luo

    Article

    Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

    Insert

    neurturin probe (Nrtn Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HCKan-P-TEF2

    Plasmid

    #65337

    Purpose

    construction of TEF2 promoter in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHL 1454

    Plasmid

    #53019

    Purpose

    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    fimB
    

    Use

    Synthetic Biology

    Tags

    RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLlac0-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3.1-R1ACR_653-EYFP

    Plasmid

    #111079

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin R1ACR_653
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSY091

    Plasmid

    #110068

    Purpose

    Bacterial expression of L4

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    L4
    

    Use

    Tags

    Expression

    Mutation

    AT-swapped mutant

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSY075

    Plasmid

    #110067

    Purpose

    Bacterial expression of L2

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    L2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AT-swapped mutant

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV5-Flag-zEPLIN

    Plasmid

    #40957

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

    Insert

    EPLIN (lima1 Zebrafish)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

39. mEos2-Talin-ABM-10

    Plasmid

    #57421

    Purpose

    Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin AMB
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Actin Binding Module; I/LWEQ = 197 amino acids fr…

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pHL 1916

    Plasmid

    #53023

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator and RBS(st7)

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only