We narrowed to 32,840 results for: Eomes

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MLM3636-Prnp-CDS-Neg

Plasmid

#61856

Purpose

Expresses a gRNA plasmid targeting the prion gene's exon 3, negative strand

Depositor

Gerold Schmitt-Ulms

Article

Mehrabian et al PLoS One. 2014 Dec 9;9(12):e114594. doi:

Insert

PrP guiding sequence (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
MEK1C218

Plasmid

#164639

Purpose

Bacterial expression plasmid for His6-MEK1C218

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S218C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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DYNLT3_pLX307

Plasmid

#98329

Purpose

Lentiviral expression of DYNLT3

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

DYNLT3 (DYNLT3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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DIMT1_pLX307

Plasmid

#98330

Purpose

Lentiviral expression of DIMT1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

DIMT1 (DIMT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEAHISPAFabH

Plasmid

#97018

Purpose

Expressing Pseudomonas aeruginosa FabH protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

3-Oxoacyl-[acyl-carrier-protein] synthase III

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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GP1BB

Plasmid

#51908

Purpose

Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

GP1BB (GP1BB Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Stop Codon at amino acid 149, before CD4, bio and…

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763)-FLAG (no His-tag)

Plasmid

#96887

Purpose

expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36

Promoter

T7

Available Since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218911

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)

Expression

Mammalian

Mutation

2363G>C and 4155C>T and 4729T>G and 4731…

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF2-E79Q

Plasmid

#207813

Purpose

N-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF2-E79Q (NFE2L2 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Mutation

E79Q

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-N SARS-CoV-2 Omicron spike protein

Plasmid

#180779

Purpose

Sars-CoV-2 variant protein

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

MAC-N SARS-CoV-2 Omicron spike protein

Tags

MAC-tag

Expression

Mammalian

Mutation

S371L, H69-V70-, S373P, S375F, G339D, N211-, N440…

Available Since

Sept. 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6P-1-MVP delta607-623aa

Plasmid

#204542

Purpose

Expresses GST-tagged MVP delta607-623aa in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

delta607-623aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R)-IRES-neo

Plasmid

#128664

Purpose

mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MEK1C218C222

Plasmid

#164640

Purpose

Bacterial expression plasmid for His6-MEK1C218C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S218C/S222C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM)-IRES-neo

Plasmid

#128671

Purpose

mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2462

Plasmid

#97064

Purpose

Expressing Sulfolobus solfataricus orf SSO2462 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2462

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2452

Plasmid

#97059

Purpose

Expressing Sulfolobus solfataricus orf SSO2452 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2452

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV119

Plasmid

#97054

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 119

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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