We narrowed to 2,568 results for: gem

1. eforRed chromoprotein

   Plasmid

   #117837

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, eforRed
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LacI-pLacI-pIV pm

   Plasmid

   #218101

   Purpose

   Phagemid that expresses LacI, and pIV under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pIV
   

   Expression

   Bacterial

   Promoter

   Lac promoter

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG4K5

   Plasmid

   #74492

   Purpose

   For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF420

   Plasmid

   #123659

   Purpose

   Produces cofactor F420 in E. coli

   Depositor

   Colin Jackson

   Article

   Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

   Inserts

   CofD
   FbiB
   FbiD
   FbiC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_R0040

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH3mCHSH2

   Plasmid

   #101053

   Purpose

   This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

   Depositor

   Jennifer Lodge

   Article

   Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

   Insert

   Cryptococcus mCherry expression plasmid
   

   Expression

   Yeast

   Promoter

   Cryptococcus Histone3 promoter

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SELP-bio-His

   Plasmid

   #51639

   Purpose

   Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SELP (SELP Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

   Plasmid

   #177265

   Purpose

   STREAMING-tag cassette

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag cassette
   

   Use

   Unspecified

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. meffBlue chromoprotein

   Plasmid

   #117845

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, meffBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. amilGFP chromoprotein

   Plasmid

   #117842

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PatoL-OG241

    Plasmid

    #72841

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoL
    

    Expression

    Bacterial

    Promoter

    PatoL

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LacI-pLacI-pII pm

    Plasmid

    #218099

    Purpose

    Phagemid that expresses LacI, and pII under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pII
    

    Expression

    Bacterial

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. meffRed chromoprotein

    Plasmid

    #117836

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffRed
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. amilGFP chromoprotein promoter-less

    Plasmid

    #118006

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. DT049A

    Plasmid

    #159410

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    tet promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. tsPurple chromoprotein promoter-less

    Plasmid

    #118007

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. promoter J23110 (upstream of RBS, mRFP1)

    Plasmid

    #117852

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    medium-high promoter, RBS, mRFP1
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pISH-Hopx

    Plasmid

    #74347

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    HOP homeobox (Hopx Mouse)
    

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Gcm2TEASY

    Plasmid

    #41031

    Depositor

    Nancy Manley

    Article

    Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

    Insert

    Gcm2 in situ probe (Gcm2 Mouse)
    

    Use

    In situ probe

    Mutation

    contains nucleotides 584-1510 of GenBank accessio…

    Promoter

    T7 sense; SP6 antisense

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only