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pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pflareG-psd95-exon18

Plasmid

#90266

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

Psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1-(151-241)

Plasmid

#24393

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 151-241 are deleted. Delta CC mutant.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-VARNAM

Plasmid

#115554

Purpose

Red fluorescent voltage indicator for neuronal expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Use

AAV

Tags

Golgi and ER export signal

Promoter

human Synapsin

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-N1

Plasmid

#54803

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pU3-gRNA

Plasmid

#53063

Purpose

Transcription of guide RNA (gRNA) in rice cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

OsU3 promoter + gRNA scaffold

Use

CRISPR; Plant expression

Promoter

rice U3

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-hGRK4

Plasmid

#32690

Depositor

Robert Lefkowitz

Article

Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

Insert

GRK4 (GRK4 Human)

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
p3990 pET ubcH8

Plasmid

#8650

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

UbcH8 (UBE2L6 Human)

Tags

His

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158651

Purpose

making transgenics expressing a UAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN_FL

Plasmid

#30223

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin without deletions (VTN Human)

Expression

Bacterial

Mutation

No deletions

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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p060 pCS mTRPS1

Plasmid

#11032

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mScarlet

Plasmid

#131000

Purpose

AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

mScarlet

Use

AAV

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBR-Lent-STAT5A-CA

Plasmid

#170662

Purpose

Lentiviral expression of constitutively active STAT5A.

Depositor

Alexander Ruthenburg

Article

Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

Insert

STAT5A (STAT5A Human)

Use

Lentiviral

Mutation

H298R and S710F

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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