We narrowed to 28,227 results for: STI;

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSiMPlc_N + pSiMPlc_C

Plasmid

#134312

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

cmR_gp41-1 N-intein + cmR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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qo5

Plasmid

#24500

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Gq alpha with the C-terminal amino acids changed …

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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qz5

Plasmid

#25867

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

D224Y

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

Plasmid

#202025

Purpose

Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)

Expression

Bacterial

Mutation

GvpB S9G-R31L-R85L

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT M87

Plasmid

#89322

Purpose

expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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