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pMM0554

Plasmid

#128990

Purpose

LEU2 integrtion vector for VP16-CIB1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Leu2 5' homology-pTDH3-SV40NLS-VP16-CIB1-tADH1-Con1-LEU2-Leu2 3' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0658

Plasmid

#129019

Purpose

pREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0557

Plasmid

#128991

Purpose

LEU2 integration vector for Zif268DBD-CRY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2-tADH1-URA3-Ura3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0558

Plasmid

#128992

Purpose

LEU2 integration vector for Zif268DBD-CRY2PHR

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2PHR-tADH1-URA3-Ura3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0657

Plasmid

#129018

Purpose

pRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0656

Plasmid

#129017

Purpose

pTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0317

Plasmid

#128979

Purpose

Encodes Zif268DBD-CRY2 under pscADH1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0320

Plasmid

#128980

Purpose

Encodes Zif268DBD-CRY2PHR under pscADH1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0629

Plasmid

#129004

Purpose

pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0521

Plasmid

#128984

Purpose

Expression plasmid for ZIF268DBD-L3-CRY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-Zif268DBD-CRY2 (L3) scTRP1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0620

Plasmid

#128995

Purpose

pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0628

Plasmid

#129003

Purpose

pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTD3685

Plasmid

#113391

Purpose

alpha factor inducible Ty3

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K127R)

Plasmid

#99543

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH3F-PYK1

Plasmid

#85774

Purpose

N-ICE plasmid pH3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW010

Plasmid

#83521

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha2

Expression

Yeast

Promoter

pARO9

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW009

Plasmid

#83523

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha1

Expression

Yeast

Promoter

pARO9

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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PHBA01

Plasmid

#83535

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC gene

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW005

Plasmid

#83524

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-malpha1

Expression

Yeast

Promoter

pFUS1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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YCp33-YDL183c-HA

Plasmid

#70164

Purpose

yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

3xHA

Expression

Yeast

Promoter

YDL183c (217 nucleotides upstream of the ATG)

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
MXS_PA-GFP

Plasmid

#62412

Purpose

MXS_chaining vector with PA-GFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PA-GFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_linker

Plasmid

#62431

Purpose

MXS_chaining vector with linker

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Linker 12AA Gly-/Ser-rich

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS4

Plasmid

#62398

Purpose

MXS_chaining vector with MCS4

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-PvuII-BglII-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1-H1017K

Plasmid

#53258

Purpose

GAL driven LexA-tagged SET1-catalytically dead (H1017K)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

H1017K mutation - catalytically dead

Promoter

GAL1/10

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBACgus N-His/GST/LRRK2 1867-2141

Plasmid

#40404

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2141

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus N-His/GST/LRRK2 1867-2161

Plasmid

#40405

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2161

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40372

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

aa1867-2161, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S

Plasmid

#40388

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pTH490-tQSRTL

Plasmid

#29699

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G

Expression

Yeast

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pWZ414-F48

Plasmid

#23072

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glutamine, H3 K14…

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F50

Plasmid

#23074

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F54

Plasmid

#23078

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO SSA1

Plasmid

#19462

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

Plasmid

#14849

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

Plasmid

#14850

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
Ypd1p-pET22b

Plasmid

#12655

Depositor

SeWon Suh

Article

Song et al J Mol Biol. 1999 Nov 5. 293(4):753-61.

Insert

YPD1 protein (YPD1 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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