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Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g2
Plasmid#80785PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. pyogenes scaffold CARGO array
Plasmid#191316PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SB_convex
Plasmid#110104PurposepBXPHM3 with scaffold Sybody of the convex libraryDepositorInsertscaffold Sybody of the convex library
UseTagsHisExpressionBacterialMutationPromoterPBADAvailable sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
P336-FBP_11
Plasmid#139702PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing LuzDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:miRFP670-cetn4
Plasmid#192357PurposezfCETN4 tagged with miRFP670, expressed under the UAS promoterDepositorInsertCETN4 (cetn4 Zebrafish)
UseTagsmiRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT001-LVB
Plasmid#163381PurposeSecond generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN systemDepositorInsertLaG17-VEEGFRtm-BFP (LVB)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
UseTags6X His and sortase A sorting motifExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hChR2(H134R)-EYFP
Plasmid#114367Purposeexpresses channelrhodopsin 2 fused with EYFPDepositorInserthChR2(H134R)
UseTagsEYFPExpressionMammalianMutationthe Histidine at amino acid position 134 was chan…PromoterpCAGAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only