We narrowed to 49,526 results for: ang
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Plasmid#167884PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Puro
Plasmid#167879PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_iRFP713
Plasmid#167876PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Hygro
Plasmid#167875PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_EYFP
Plasmid#167874PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Blasto
Plasmid#167872PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc397
Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN216
Plasmid#167963Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN217
Plasmid#167964Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN262
Plasmid#167967Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
UseTagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
UseTagshexa-histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN202
Plasmid#167950Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmx-puro-Mef2c-i
Plasmid#172398PurposeRetroviral expression vector for murine direct cardiac reprogrammingDepositorInsertMef2c-i (Mef2c Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1 Y523F
Plasmid#124373PurposeExpresses PSPC1 Y523F in mammalian cellsDepositorInsertPSPC1 (PSPC1 Human)
UseTagsHA and HA tagExpressionMammalianMutationY523FPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shSpc25
Plasmid#160960PurposeSpc25 shRNA in pMKO.1 retroviral vectorDepositorInsertSpc25 shRNA (Spc25 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(G:H)
Plasmid#138259PurposeExpression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter systemDepositorInsertmCherry targeting sgRNAs G and H
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati Exons 1-3
Plasmid#110113PurposeDrosophila dati minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mECitrine
Plasmid#105152PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mECitrine
UseTags41nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter41nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C71,105A
Plasmid#58394PurposeExpressed murine IFITM3-C71,105A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 71 and 105 to AlaninePromoterCMV IEAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C72,105A
Plasmid#58395PurposeExpressed murine IFITM3-C72,105A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 72 and 105 to AlaninePromoterCMV IEAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C71,72A
Plasmid#58393PurposeExpressed murine IFITM3-C71,72A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 71 and 72 to AlaninePromoterCMV IEAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C72A
Plasmid#58391PurposeExpressed murine IFITM3-C72A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 72 to AlaninePromoterCMV IEAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C71A
Plasmid#58390PurposeExpressed murine IFITM3-C71A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 71 to AlaninePromoterCMV IEAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab8 DN
Plasmid#58451Purposeexpression of drosophila YFP Rab8 dominant negative mutantDepositorInsertRab8 (Rab8 Fly)
UseTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 DN
Plasmid#58445Purposeexpression of drosophila YFP-Rab2 dominant negative mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 CA
Plasmid#58443Purposeexpression of drosophila YFP-Rab2 constitutively active mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 DN
Plasmid#37715DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS49N (dominant negative)PromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
Mm-PylRS-AF/Pyl-tRNACUA
Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
UseTagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…PromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Oct4-IRES-eGFP-PGK-Neo
Plasmid#48681PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertOct4 (Pou5f1 Mouse)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2-WPRE
Plasmid#218880PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRV
Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-2A-ZsGreen
Plasmid#231985PurposeEmpty backbone for expression of GoI with Bi-cistronic expression ZsGreenDepositorInsert2A-ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-CAG-Flpe-ERT2
Plasmid#68460PurposeAAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection geneDepositorInsertmammalian-codon-optimized Flpe recombinase fused with ERT2
UseSynthetic BiologyTagsERT2ExpressionMammalianMutationPromoterCAGAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX301
Plasmid#25895DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
p35S:Citrine-TurboID-3xHA
Plasmid#199243PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID
UseTags3xHA and TurboID-3xHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX302
Plasmid#25896DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUBQ:Citrine-TurboID-3xMyc
Plasmid#199244PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID-3xMyc
UseTagsTurboID-3xMycExpressionPlantMutationPromoterArabidopsis UBQ10 promoter with intronAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag
Plasmid#132411Purposeoverexpression in human cellsDepositorInsertdRfxCas13b
UseLentiviralTagsflagExpressionMammalianMutationR239A; H244A; R858A; H863APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-iCasp9
Plasmid#199441PurposeExpress inducible Caspase9 in mammalian cellsDepositorInsertinducible Caspase9 (CASP9 Human)
UseLentiviralTagsDmrB and mCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dual-CRISPR-U6-H1(pBP43)
Plasmid#218930PurposeThe backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 and H1Available sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only