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Addgene

We narrowed to 49,526 results for: ang

Showing: 6301 - 6350 of 49526 results
  1. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pBN216

    Plasmid
    #167963
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Depositor
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pBN217

    Plasmid
    #167964
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Depositor
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pBN262

    Plasmid
    #167967
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Depositor
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pBRC901

    Plasmid
    #167948
    Purpose
    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Depositor
    Insert
    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pBN202

    Plasmid
    #167950
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Depositor
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pRWJB004

    Plasmid
    #160112
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pUC19-hsg(G:H)

    Plasmid
    #138259
    Purpose
    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
    Depositor
    Insert
    mCherry targeting sgRNAs G and H
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. p3.2mar(T->G)-GFP

    Plasmid
    #63695
    Purpose
    3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporter
    Depositor
    Insert
    3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    T->G bp change
    Promoter
    hsp70
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Insert
    PylRS-AF, Pyl-tRNACUA
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Promoter
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid
    #218874
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    Synapsin
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 6301 - 6350 of 49526 results