We narrowed to 6,005 results for: 338

1. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Human, Synthetic)
   

   Use

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST_116_LVL2 cam

   Plasmid

   #179332

   Purpose

   NT-CRISPR plasmid for a single gRNA.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Rtn4b-GFP

   Plasmid

   #186952

   Purpose

   Expresses Rtn4b connected with C-terminus AcGFP1

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   RTN4B (RTN4 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX_lenti_osTir1_9Myc_P2A_Bsd

   Plasmid

   #139746

   Purpose

   lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   rice E3 ligase Tir1 (osTir1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 Flag beta-1-adrenergic-receptor

   Plasmid

   #14698

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B1AR (ADRB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWPI-SLC7A11-HA

   Plasmid

   #201643

   Purpose

   Over-expression of HA-tagged human SLC7A11

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Sabrina Spencer, Tobias Meyer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-EGFP-RhoA-T19N

    Plasmid

    #12967

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA DN (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T19N Dominant negative

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MuHKII-pGFPN3

    Plasmid

    #21922

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKII cDNA sequence …

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. TrHKII-pGFPN3

    Plasmid

    #21921

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Truncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the truncated human HKII cDNA sequence (l…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pETM-41-HsPABPC1_J

    Plasmid

    #146644

    Purpose

    Bacterial Expression of HsPABPc1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsPABPc1 (PABPC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLNCX2-mEGFP-CHMP2B

    Plasmid

    #115329

    Purpose

    expresses mEGFP-CHMP2B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ntn4-Fc-His

    Plasmid

    #72106

    Purpose

    Expresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn4 (Ntn4 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Unc5b.z-AP-His

    Plasmid

    #72053

    Purpose

    Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b.z (Unc5b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. 3xFLAG-CUL3-pCMV7.1

    Plasmid

    #155021

    Purpose

    N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 3 (CUL3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM60 FLAG-RAB35 S22N

    Plasmid

    #107106

    Purpose

    Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

    Depositor

    Charles Sawyers

    Article

    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pv6_TAF3_2xPhd

    Plasmid

    #179399

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    TAF3_2xPhd
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge