We narrowed to 5,339 results for: 290

1. pET28a LZ control-FLAG

   Plasmid

   #199678

   Purpose

   Bacterial expression of Leucine Zipper (LZ) control-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper control
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-1-ε ear

   Plasmid

   #196670

   Purpose

   Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 ε ear (840-1137) (AP4E1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   silent mutations in codons 905, 1129 and 1137

   Promoter

   tac

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1-TSF-ε

   Plasmid

   #196583

   Purpose

   Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 ε subunit (AP4E1 Human)
   

   Use

   Tags

   two Strep/one FLAG (TSF)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2869

   Plasmid

   #144160

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF865 (ZNF865 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

   Plasmid

   #186703

   Purpose

   Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV Hsd11b1-V5

   Plasmid

   #175147

   Purpose

   Lentiviral expression of mouse Hsd11b1-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Hsd11b1 (Hsd11b1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20 CARD8-HA PAM L368G

   Plasmid

   #169985

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, L368G

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer20 CARD8-HA PAM R394E

   Plasmid

   #169987

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, R394E

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pInducer20 CARD8-HA PAM F405G

   Plasmid

   #169988

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F405G

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pInducer20 CARD8-HA PAM F370G

    Plasmid

    #169986

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F370G

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 CARD8 FIIND F405G + S297A

    Plasmid

    #169974

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, F405G

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 CARD8 FIIND E274R + S297A

    Plasmid

    #169968

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, E274R

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 CARD8 FIIND R394E + S297A

    Plasmid

    #169972

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, R394E

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 CARD8 PAM E274R

    Plasmid

    #169977

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    PAM, E274R

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

    Plasmid

    #169955

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

    Plasmid

    #169957

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, F405G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

    Plasmid

    #169951

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R, S297A

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM10 with rat Δ192Annexin A11

    Plasmid

    #164498

    Purpose

    For bacterial expression of rat His-tagged Δ192Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ192Annexin A11 (Anxa11 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM10 with rat Δ188Annexin A11

    Plasmid

    #164497

    Purpose

    For bacterial expression of rat His-tagged Δ188Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ188Annexin A11 (Anxa11 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDB.His.MBP.3C CARD8 CARD A452C R459E

    Plasmid

    #164024

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    A452C, R459E

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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