We narrowed to 7,211 results for: CIT

1. xCas9(3.7)-ABE(7.10)

   Plasmid

   #108382

   Purpose

   Mammalian expression vector for xCas9(3.7)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-ABE(7.10)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

   Plasmid

   #89149

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mNeptune2.5

   Plasmid

   #51310

   Purpose

   Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2.5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETa-mMaple3-V157I

   Plasmid

   #98577

   Purpose

   photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mMaple3-V157I
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AY27_pU6.gRNA.CLYBL

   Plasmid

   #199238

   Purpose

   Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. pyogenes gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-Ub KO

   Plasmid

   #11934

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin KO (RBX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   All 7 lysines of ubiquitin mutated to arginines.

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNX29-PX (269-391)

   Plasmid

   #119108

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX29-PX (269-391)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-OGT_opt

   Plasmid

   #190858

   Purpose

   For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells.

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CMV PGK1-3xHA

    Plasmid

    #220913

    Purpose

    Expresses PGK1-3xHA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-3xHA (PGK1 Human)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SNX10-PX (1-201)

    Plasmid

    #119091

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX10-PX (1-201) (SNX10 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-synaptoPAC-minWPRE

    Plasmid

    #153085

    Purpose

    Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

    Depositor

    Dietmar Schmitz

    Article

    Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

    Inserts

    bPAC(WT)
    Synaptophysin (Syp Rat)
    

    Use

    AAV

    Tags

    His tag, mScarlet, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUB1 (full-length, aa 1-271)

    Plasmid

    #61420

    Purpose

    Expresses human OTUB1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. ETV5_pet28a

    Plasmid

    #131639

    Purpose

    Bacterial expression of ETS gene ETV5

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV5 (ETV5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ265E2

    Plasmid

    #164719

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ELK1_pet28a

    Plasmid

    #131647

    Purpose

    Bacterial expression of ETS gene ELK1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. dxCas(3.7)-VPR

    Plasmid

    #108383

    Purpose

    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.7)-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-Flpo

    Plasmid

    #183535

    Purpose

    INTRSECT

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CaMKIIa

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a core

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. VN-beta Synuclein

    Plasmid

    #89483

    Purpose

    expresses human beta Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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