We narrowed to 40,035 results for: KAN;

1. pRKO

   Plasmid

   #49456

   Purpose

   loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

   Depositor

   Marc Gartenberg

   Article

   Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

   Insert

   loxP-kanMX-loxP cassette
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF/myc/ER FLII12Pglu-700uDelta6

   Plasmid

   #17867

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLII12Pglu-700uDelta6
   

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   700uDelta6, ECFP insert in mglB

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK377 (Hygro-P2A-mCherry2)

   Plasmid

   #121188

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mCherry2
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTJV1ScKm-rpoB

   Plasmid

   #166980

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   NeoR/KanR
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. nuc-gTEMP_pcDNA3

   Plasmid

   #109118

   Purpose

   Nucleus targetting gTEMP

   Depositor

   Takeharu Nagai

   Article

   Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

   Insert

   nuc-gTEMP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK408 (mAID-Nluc Hygro)

   Plasmid

   #121198

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   mAID-Nluc Hygro
   

   Use

   CRISPR

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-p40R57Q PX

   Plasmid

   #19009

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa1-148 (PX domains) site directed mutagenesis: …

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pBSFI-p110β

   Plasmid

   #34891

   Depositor

   Peter Vogt

   Article

   Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

   Insert

   p110β (PIK3CB Human)
   

   Use

   Adaptor plasmid

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-DEST-hSOX2-EGFP-PuroTK

   Plasmid

   #141026

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK394 (mAID-Nluc-Neo)

    Plasmid

    #121195

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-Nluc-Neo
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoHA mouse Numb

    Plasmid

    #37012

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    Numb (Numb Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains Numb aa 8-853

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMK376 (Hygro-P2A-mClover)

    Plasmid

    #121187

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. P16ΔS:GFP1–9

    Plasmid

    #108186

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    GFP1-9
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    P16ΔS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIneoMyc mouse Numb

    Plasmid

    #41712

    Depositor

    Yutaka Hata

    Article

    Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

    Insert

    Numb (Numb Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    contains amino acids 8-853

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pUC-DEST-R1R2

    Plasmid

    #141056

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG25-L-IFP-F[1] (clon 3)

    Plasmid

    #52899

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Vector for homologous recombination

    Promoter

    TEF promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAG32-L-IFP-F[2] (clon 3)

    Plasmid

    #52900

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Vector for homologous recombination

    Promoter

    TEF promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pUC-DEST-R3R4(R)

    Plasmid

    #141010

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9–mKate2ps–T1gRNA

    Plasmid

    #62717

    Purpose

    t1 gRNA (ATGAGAATCAAGGCGGTCGA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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