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pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMX241

Plasmid

#23017

Depositor

Linda Wordeman

Article

Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

Insert

GCPBMhyp

Tags

CenpB 1-167 and EGFP

Expression

Mammalian

Mutation

deleted MCAK amino acids 1-178, 584-718 mutated …

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAH3

Plasmid

#41827

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

Depositor

Melissa Moore

Article

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Yeast

Promoter

TEF

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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Rv2002 M3/pET-28b(+)N-tag

Plasmid

#13057

Depositor

SeWon Suh

Article

Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

Insert

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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L1p1_min35S_Luciferase

Plasmid

#231035

Purpose

Cloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOS

Depositor

Wilma van Esse

Article

Winkelmolen et al bioRxiv 2024.08.22.609225

Insert

Minimal p35S:Luciferase:tNOS

Use

Luciferase

Expression

Bacterial

Promoter

minimal p35S

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCOLA-Gent-EV

Plasmid

#202491

Purpose

pCOLA-Gent Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-N-GFP

Plasmid

#222602

Purpose

Expression of MAC3-tagged GFP (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP

Plasmid

#222601

Purpose

Expression of MAC3-tagged GFP (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-1_ZF1

Plasmid

#213844

Purpose

CombiGEM storage plasmid for ZF-1 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-2_ZF2

Plasmid

#213845

Purpose

CombiGEM storage plasmid for ZF-2 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLEU

Plasmid

#213846

Purpose

LEU integrating plasmid backbone used to create all of the reporter plasmids.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-ChroMEd15-mTFP

Plasmid

#184240

Purpose

channelrhodopsin ChroME fused to mTFP

Depositor

Yiyang Gong

Article

Beck et al Biophys J. 2022 Mar 21. pii: S0006-3495(

Insert

ChroMEd15-mTFP

Use

Lentiviral

Tags

mTFP1

Expression

Mammalian

Mutation

deleted ChroME amino acids 317-331

Promoter

Camk2

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0M7LBC1

Plasmid

#163181

Purpose

Inducible expression of UniProt identifier A0A0M7LBC1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0M7LBC1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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