We narrowed to 22,637 results for: CRISPR

1. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD_TypeIC_Cascade_cas3_invivo

   Plasmid

   #196400

   Purpose

   expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Inserts

   Cas5c
   Cas7c
   Cas8c
   Cas3c
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

   Plasmid

   #64512

   Purpose

   Expression of St1 dCas9-3XTagBFP2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnCasPA-BEC

    Plasmid

    #113349

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDestTol2CG2-U6:gRNA

    Plasmid

    #63156

    Purpose

    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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