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pIA1400

Plasmid

#172519

Purpose

Expression of SARS-COV-2 Nsp12 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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NODAL-matMYC

Plasmid

#115248

Purpose

For mammalian expression of the human NODAL open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP

Plasmid

#138657

Purpose

stable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yeGFP

Use

Yeast reporter plasmid

Promoter

CYC1

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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yfpsyndat

Plasmid

#19991

Depositor

Jonathan Javitch

Article

Carvelli et al J Neurochem. 2002 May . 81(4):859-69.

Insert

yfpsynhdat (SLC6A3 Human)

Tags

yfp

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Tags

3xFlag, His, and T7

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
His-tagged-SF1

Plasmid

#28182

Depositor

Bernard Schimmer

Article

Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

Insert

SF1 (Nr5a1 Mouse)

Tags

His

Expression

Mammalian

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pIA1364

Plasmid

#172401

Purpose

Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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FLM-Q4225P

Plasmid

#41582

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM Q4225P

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII-MBII85 probe

Plasmid

#67646

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Use

For in vitro transcription

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pIA1405

Plasmid

#172404

Purpose

Expression of SARS-COV-2 Nsp12 D218A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D218A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1420

Plasmid

#172406

Purpose

Expression of SARS-COV-2 Nsp12 R116A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

R116A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1421

Plasmid

#172407

Purpose

Expression of SARS-COV-2 Nsp12 K50A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1402

Plasmid

#172403

Purpose

Expression of SARS-COV-2 Nsp12 Y129A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

Y129A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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FLM-R4136G

Plasmid

#41579

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM R4136G

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-R4154C

Plasmid

#41580

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM R4136G

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-S4190F

Plasmid

#41581

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM S4190F

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only