Addgene

We narrowed to 13,344 results for: Lor;

Showing: 241 - 260 of 13344 results

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J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23110-mRFP1-331Bb

Plasmid

#78277

Purpose

mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23110-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23103-mRFP1-331Bb

Plasmid

#78273

Purpose

mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23103-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23105-mRFP1-331Bb

Plasmid

#78275

Purpose

mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23105-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23101-mRFP1-331Bb

Plasmid

#78272

Purpose

mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23101-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461(E164D)(43-204)

Plasmid

#42508

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Deleted amino acids 1-42 (nucleotide 1-126); chan…

Promoter

tac

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
csd2_121-308,S166A/pET28b(+)

Plasmid

#83301

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Serine 166 …

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,T151A/pET28b(+)

Plasmid

#83300

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Threonine 1…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,Y197F/pET28b(+)

Plasmid

#83299

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Tyrosine 19…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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csd2_121-308,E165A/pET28b(+)

Plasmid

#83298

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120, and changed Glutamic ac…

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Expression

Mutation

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMT[Hygro]_GBP-3xFlag-DEST

Plasmid

#157813

Purpose

A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

Depositor

Zoltan Lipinszki

Article

Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

Insert

GFP-binding protein

Use

For drosophila cells

Tags

3xFlag

Expression

Insect

Mutation

Promoter

metallothionein

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a Hp-iPGM-bio

Plasmid

#179615

Purpose

Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

H. pylori iPGM bio-6His

Use

Tags

His tag and biotinylation sequence

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Rat, Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Use

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

Plasmid

#208048

Purpose

Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

BirAopt

Use

Lentiviral

Tags

Expression

Mutation

BirAopt is human-codon-optimized BirA (PMID 15707…

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Expression

Mutation

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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