We narrowed to 9,215 results for: Pol

1. Lenti-sgPole#1/Cre

   Plasmid

   #173609

   Purpose

   Expresses a Pole-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pole (Pole Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-POLBHR-eGFP

   Plasmid

   #176064

   Purpose

   Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   EGFP-PolB (POLB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   pUC with endogenous SapI mutated out, PAM mutatio…

   Promoter

   N/A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiGuide-PolB1-puro

   Plasmid

   #177146

   Purpose

   Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolBgRNA1 (POLB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27Linear-2, PolyU(0)

   Plasmid

   #166994

   Purpose

   Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0)
   

   Expression

   Mammalian

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-U6+27-Linear-1, PolyU(0)

   Plasmid

   #166992

   Purpose

   Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GLY, Linear-3, PolyU(1)

   Plasmid

   #166988

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GLY, Linear-3, PolyU(4)

   Plasmid

   #166989

   Purpose

   Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GLN, Linear-3, PolyU(4)

   Plasmid

   #166985

   Purpose

   Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4)
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-AN-DDK_S433A POLI

   Plasmid

   #131235

   Purpose

   Mammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-DDK_S497A POLI

    Plasmid

    #131236

    Purpose

    Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_S520A POLI

    Plasmid

    #131237

    Purpose

    Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_S580A POLI

    Plasmid

    #131238

    Purpose

    Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-DDK_3KA POLI

    Plasmid

    #131239

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert lysine resi…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGST-pol1-N2-2A2

    Plasmid

    #160991

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGST-pol1-N1-2A2

    Plasmid

    #160990

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-U6+27-Linear-1_PolyU(1)

    Plasmid

    #159479

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(1)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV-U6+27-Linear-1_PolyU(2)

    Plasmid

    #159480

    Purpose

    Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(2)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-U6+27-Linear-1_PolyU(3)

    Plasmid

    #159481

    Purpose

    Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-1_PolyU(3)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmiRGLO-POLR2E-213

    Plasmid

    #117356

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR POLR2E-213
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. YCe1889 HC_Kan_SV40-polyA_p11

    Plasmid

    #100678

    Purpose

    part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    SV40-polyA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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