We narrowed to 4,104 results for: plasmid lenti crispr

1. pLenti_PEmax-2A-MLH1dn-2A-BSD

   Plasmid

   #234072

   Purpose

   For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax-2A-MLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LentiAsCpf1-3xMYC-blast

   Plasmid

   #127750

   Purpose

   Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Insert

   AsCpf1
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-hU6-cs1-PuroR-T2A-BFP

   Plasmid

   #230934

   Purpose

   gRNA cloning vector with direct capture sequence 1 in stem loop 2

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

   Plasmid

   #170378

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   human Synapsin promoter

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

   Plasmid

   #230935

   Purpose

   gRNA cloning vector encoding BFP and puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Promoter

   hU6

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-NRCH-PE2-BSD

   Plasmid

   #191101

   Purpose

   Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PE2-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. plenti-Lb-Cas12a-2xNLS

   Plasmid

   #155046

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-NRCH-PE2max-BSD

    Plasmid

    #191103

    Purpose

    Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    NRCH-PEmax
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9-NRCH

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiRCas9-Lambda2

    Plasmid

    #104184

    Purpose

    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-sgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiUniversal-Puro

    Plasmid

    #127749

    Purpose

    Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

    Depositor

    Giulio Draetta

    Article

    Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiGuide Puro-P2A-EGFP

    Plasmid

    #137729

    Purpose

    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOT_7-lenti-EFS-SS1-BBz-2A-puro

    Plasmid

    #217990

    Purpose

    Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Xue et al bioRxiv 2024.11.27.625752

    Insert

    SS1-BBz CAR
    

    Use

    Lentiviral

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Mutation

    D10A and H840A

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge