We narrowed to 2,671 results for: prep

1. pJYDNg

   Plasmid

   #162452

   Purpose

   Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   Ha and myc and eUnaG2 fluorescent protein

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-deadMalibu-mScarlet

   Plasmid

   #229555

   Purpose

   Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

   Depositor

   Danielle Schmitt

   Article

   Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

   Insert

   Malibu-mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPInt-Dendra2-Nat

   Plasmid

   #236494

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYY62-P1int-TP901-GFPdropout

   Plasmid

   #235773

   Purpose

   Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

   Depositor

   Chang Liu

   Article

   Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZZMP09

   Plasmid

   #231754

   Purpose

   Mammalian expression of SPO11

   Depositor

   Scott Keeney

   Article

   Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

   Insert

   SPO11 (Spo11 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_NKX2-5

   Plasmid

   #221479

   Purpose

   Gateway cloning of NKX2-5 overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-5 (NKX2-5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJYDNp

   Plasmid

   #162451

   Purpose

   Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-eUnaG2
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA and myc

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJYDC3

   Plasmid

   #162460

   Purpose

   Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RCAS(A) mdnTcf4Ben (CT#1130)

   Plasmid

   #14020

   Depositor

   Cliff Tabin

   Article

   Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

   Insert

   Tcf4B (Tcf7l2 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Dominant negative Tcf4B, engrailed fusion

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pJYDC2

    Plasmid

    #162459

    Purpose

    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMON-HsNMT2

    Plasmid

    #67476

    Purpose

    Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT2 (NMT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBAD24-ALFA-TwinStrep

    Plasmid

    #240248

    Purpose

    Arabinose-inducible plasmid with ALFA and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al bioRxiv [Preprint]. 2024 Apr 25:2023.12.

    Type

    Empty backbone

    Use

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-ALFA-TwinStrep

    Plasmid

    #240252

    Purpose

    IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al bioRxiv [Preprint]. 2024 Apr 25:2023.12.

    Type

    Empty backbone

    Use

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_CKAR3

    Plasmid

    #238411

    Purpose

    Mammalian expression of CKAR3 under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

    Insert

    CKAR3 (PKC sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5_mutCKAR3

    Plasmid

    #238412

    Purpose

    Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

    Insert

    phosphorylation deficient mutant of CKAR3 (T to A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPInt-mStayGold-Hyg

    Plasmid

    #236486

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPInt-mStayGold-Nat

    Plasmid

    #236485

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAPInt-mCherry-Nat

    Plasmid

    #236488

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPInt-sfGFP-Hyg

    Plasmid

    #236480

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAPInt-mScarlet-Nat

    Plasmid

    #236491

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge