We narrowed to 3,145 results for: N-EGFP

1. Lyn11-CIBN-GFP

   Plasmid

   #79572

   Purpose

   expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   Lyn11
   

   Use

   Tags

   CIBN (CRY2-binding domain) and EGFP

   Expression

   Mammalian

   Mutation

   G8R (please see depositor comments below)

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

   Plasmid

   #199350

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

   Plasmid

   #199348

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pStr-KDEL_APV-SBP-DsRed2-ER-5

   Plasmid

   #170675

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   APV-SBP-DsRed2-ER-5
   streptavidin-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

   Plasmid

   #199347

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pStr-KDEL_EPV-SBP-DsRed2-ER-5

   Plasmid

   #170676

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   EPV-SBP-DsRed2-ER-5
   streptavidin-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7RNAPNev-NB1

   Plasmid

   #216336

   Purpose

   Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

   Depositor

   Mark Styczynski

   Article

   McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

   Insert

   T7RNAPN-NB1 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LPUtopia-8

   Plasmid

   #224781

   Purpose

   Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   puromycin N-acetyltransferase
   enhanced Green Fluorescence Protein
   Red Fluorescence Protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pStr-KDEL_APV-SBP-Dendra2-ER-5

   Plasmid

   #170679

   Purpose

   Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Inserts

   APV-SBP-Dendra2-ER-5
   streptavidin-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hiActTS-GR

    Plasmid

    #79775

    Purpose

    high FRET control for ActTS-GR

    Depositor

    Tetsuya Kitaguchi

    Article

    Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

    Insert

    hiActTS-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ActTS-GR

    Plasmid

    #79774

    Purpose

    FRET-based actinin tension sensors

    Depositor

    Tetsuya Kitaguchi

    Article

    Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

    Insert

    ActTS-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSMALB-ATF4.12

    Plasmid

    #155033

    Purpose

    Fluorescent reporter for ATF4 translation, Negative Control

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    uORF1 start codon mutated

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMALB-ATF4.14

    Plasmid

    #155034

    Purpose

    Fluorescent reporter for ATF4 translation, Positive Control

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    uORF1 and uORF2 start codons mutated

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LPUtopia-7

    Plasmid

    #199212

    Purpose

    Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Inserts

    aminoglycoside phosphotransferase,
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and thymidine kinase promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-ubiquitin-EphB2-SV40-GFP (B2G)

    Plasmid

    #65442

    Purpose

    Expression of mouse EPHB2 and GFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    EPHB2 (Ephb2 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-EGFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    UBQ

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB-CG-LYCHOS WT-HA-GFP

    Plasmid

    #199687

    Purpose

    Expression of LYCHOS WT in insect cells

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Use

    Tags

    10xHis, EGFP, HA, HRV 3C, and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMS2-YFP

    Plasmid

    #27122

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Use

    Tags

    EYFP, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CBF:H2B-Venus

    Plasmid

    #44211

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

    Insert

    CBF1-Responsive-Element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-GCaMP6fu

    Plasmid

    #86235

    Purpose

    Express fluorescent calcium indicator GCaMP6fu in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

    Insert

    GCaMP6fu
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W43Y D395A

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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