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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,724 results for: gem

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Showing: 2581 - 2600 of 2724 results

  1. pTARGEX-CHL

    Plasmid
    #242447
    Purpose
    Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    Transit peptide from N. tabacum RbcS
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242447-plasmid-map-sequence-id-490473

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  2. pTARGEX-APO

    Plasmid
    #242446
    Purpose
    Targets high levels of recombinant protein to apoplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    N-terminal fusion with PR1b signal peptide
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242446-plasmid-map-sequence-id-490468

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  3. pTARGEX-ER

    Plasmid
    #242449
    Purpose
    Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    ER-retrieval motif (KDEL) and Signal peptide deri…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242449-plasmid-map-sequence-id-489545

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  4. SMT503 - S77A

    Plasmid
    #226842
    Purpose
    Plasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. Chloramp
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Lipase A
    Use
    Synthetic Biology
    Tags
    SNAP and eGFP
    Expression
    Bacterial
    Mutation
    R33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157M
    Promoter
    T7
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    226842-plasmid-map-sequence-id-456036

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  5. SMT503

    Plasmid
    #226840
    Purpose
    Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Lipase A
    Use
    Synthetic Biology
    Tags
    SNAP and eGFP
    Expression
    Bacterial
    Mutation
    R33Q, D34N, K35D, K112D, M134D, Y139C, I157M
    Promoter
    T7
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    226840-plasmid-map-sequence-id-456035

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  18. DT048A

    Plasmid
    #159409
    Purpose
    Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23101-LuxR-pr.LuxR-PFO
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG
    Expression
    Bacterial
    Promoter
    J23101 and pLux promoter
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159409-plasmid-map-sequence-id-326577

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  19. DT046A

    Plasmid
    #159407
    Purpose
    Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.tet-LuxR-pr.LuxR-PFO
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG
    Expression
    Bacterial
    Promoter
    tet and pLux promoter
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159407-plasmid-map-sequence-id-326569

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  20. DT047A

    Plasmid
    #159408
    Purpose
    Constitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-LuxR-pr.LuxR-sfGFP-PFO
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG
    Expression
    Bacterial
    Promoter
    tet and pLux promoter
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159408-plasmid-map-sequence-id-326571

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Showing: 2581 - 2600 of 2724 results