We narrowed to 5,214 results for: GST

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pJLI-Sup35N-KDG6-C

Plasmid

#1238

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Use

Yeast integrative plasmid

Mutation

SUP35 middle region (aa124-253) was replaced with…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Sc NybbR eRF3∆165N (H348E)

Plasmid

#175420

Purpose

Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N, H348E

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJC25R1+4stop

Plasmid

#17723

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Expression

Bacterial

Mutation

E.coli expression plasmid for NM-region of Sup35 …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 Q331K

Plasmid

#27465

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 M337V

Plasmid

#27464

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pDuet TDP-43 G294A

Plasmid

#27463

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-eRF3a F76a

Plasmid

#65811

Purpose

expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

eRF3a (GSPT1 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

F76a

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmSTING

Plasmid

#208386

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgSTING (Sting1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDCAF3-IS5

Plasmid

#65220

Purpose

Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

Depositor

Jeffrey Barrick

Article

Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

Inserts

ndmA
ndmB
ndmC
ndmD
gst9
chlR

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed start codon from gtg to atg and Changed s…

Promoter

BBa_J23100

Available Since

Jan. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX GAC F7

Plasmid

#227829

Purpose

To express in E. coli GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

GAC isoform of F7 (GLS Human)

Tags

GST

Expression

Bacterial

Mutation

P137, L139, E140, L142, Y145, G150, Q151, E152, K…

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX KGA F7

Plasmid

#227822

Purpose

To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

KGA isoform of F7 (GLS Human)

Tags

GST

Expression

Bacterial

Mutation

P137, L139, E140, L142, Y145, G150, Q151, E152, K…

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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