We narrowed to 9,260 results for: Pol

1. pVITRO1-102.1F10-IgG3/λ

   Plasmid

   #50368

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. eDrHER2

   Plasmid

   #183995

   Purpose

   Optically controlled eDrHER2 expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrHER2 (ERBB2 Human)
   

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-IpgD(C439S)

   Plasmid

   #183667

   Purpose

   N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C439S

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDTFendoNA2

   Plasmid

   #174731

   Purpose

   Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.

   Depositor

   Jukka Finne

   Article

   Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

   Insert

   DT386F
   

   Expression

   Bacterial

   Mutation

   Deleted amino acids 412-560 (the receptor binding…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. G31, Cdc20-AID tagging

   Plasmid

   #130269

   Purpose

   Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

   Depositor

   Matthias Heinemann

   Article

   Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

   Insert

   Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
   

   Tags

   AID(71-114)

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SopB-EGFP

   Plasmid

   #183655

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-LEU-SopB

   Plasmid

   #183670

   Purpose

   Inducible Salmonella Typhimurium SopB for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pESC-Leu-SopB(C460S)

   Plasmid

   #183671

   Purpose

   Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Mutation

   C460S

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-SRGAP2A-FBAR-mRFP

   Plasmid

   #168502

   Purpose

   Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Insert

   SRGAP2A-FBAR (SRGAP2 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_SRGAP2A_SH3(W765A)_GFP

    Plasmid

    #222616

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_SRGAP2A_SH3(W765A)_HA

    Plasmid

    #222614

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG SRGAP2A_EVH1(P339L F342C)

    Plasmid

    #222613

    Purpose

    Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutations P339L and F342C

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet28b_His_RAD23A-UBA1

    Plasmid

    #201549

    Purpose

    Expresses UBA1 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 160-205 of DNA polymerase i…

    Promoter

    T7

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28b-His_RAD23A-UBA2

    Plasmid

    #201550

    Purpose

    Expresses UBA2 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 311-363 of DNA polymerase i…

    Promoter

    T7

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-SopB(K525A)

    Plasmid

    #183659

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K525A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-SopB(K528A)

    Plasmid

    #183660

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K528A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-SopB(33-554)

    Plasmid

    #183661

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 33-554

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-SopB(33-520)

    Plasmid

    #183662

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 33-520

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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