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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBT3035

Plasmid

#122543

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Article

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Tags

GFP and Avi

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_2xChromo-13XL-BASU

Plasmid

#179413

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.2xChromo-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSRW1JZ

Plasmid

#129528

Purpose

C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

Depositor

Noelle L'Etoile

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMB71

Plasmid

#73085

Purpose

Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-Y530EEI

Plasmid

#140323

Purpose

Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pv9_MBD1_MBD-WT-13XL-BASU

Plasmid

#179416

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1.MBD-WT-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ889

Plasmid

#84826

Purpose

Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

smu-1 (smu-1 Nematode)

Tags

GFP

Expression

Bacterial and Worm

Promoter

Psmu-1

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD

Plasmid

#140304

Purpose

Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-KD

Plasmid

#140296

Purpose

Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD-KD

Plasmid

#140300

Purpose

Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

lacks the SH3 and SH2 domains

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Tags

BC2-tag and Ypet

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-VIM-BC2T

Plasmid

#108966

Purpose

expresses mCherry-vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

VIM (VIM Human)

Tags

BC2T and mCherry

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pOD2049-csnControl

Plasmid

#100007

Purpose

MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Inserts

zif-1 (zif-1 Nematode)
unc-119

Use

Targeting vector for mos1 transposon mediated sin…

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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