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pGEX Grb2-SH2

Plasmid

#46440

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK11-G56W

Plasmid

#116687

Purpose

Lentiviral expression of STK11 G56W

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK11 (STK11 Human)

Use

Lentiviral

Mutation

G56W

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _60-89

Plasmid

#59144

Purpose

YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

aa60-89 deleted

Promoter

CMV

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK19

Plasmid

#116795

Purpose

Lentiviral expression of STK19

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK19 (STK19 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _PDZ

Plasmid

#59147

Purpose

YAP1 mutants that harbored a deletion of the 5 amino acids

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ binding domain (last 5aa) deleted

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-RCAN2

Plasmid

#116786

Purpose

Lentiviral expression of RCAN2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RCAN2 (RCAN2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK33

Plasmid

#116797

Purpose

Lentiviral expression of STK33

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK33 (STK33 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc2/C - AVITEV

Plasmid

#74058

Purpose

retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Abl-SH2

Plasmid

#46402

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl (ABL1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 120-225

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _SH3bm

Plasmid

#59141

Purpose

YAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

SH3 domain deleted (aa278-290)

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc3-Mdm2 p60

Plasmid

#52059

Purpose

expression of truncated Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 p60 (truncated) (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

p60 (truncated at D367, DVDP motif)

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-PRKD1

Plasmid

#116778

Purpose

Lentiviral expression of PRKD1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PRKD1 (PRKD1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK31

Plasmid

#116796

Purpose

Lentiviral expression of STK31

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK31 (STK31 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Btk-SH2

Plasmid

#46412

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Btk (BTK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 271-385

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hH4-AVITEV

Plasmid

#74051

Purpose

retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Histone-H4 (H4C16 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd6

Plasmid

#102868

Purpose

Expresses mouse Fzd6 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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