We narrowed to 18,413 results for: multi

1. pCRISPomyces-1

   Plasmid

   #61736

   Purpose

   Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   tracrRNA
   crRNA cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR- flag-Lag17-iCAM-1

   Plasmid

   #205204

   Purpose

   This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and Lag17 anti-GFP nanobody
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330A_D10A-1x5

   Plasmid

   #58775

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL tension sensor module

   Plasmid

   #101170

   Purpose

   Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

   Depositor

   Carsten Grashoff

   Article

   Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

   Insert

   YPet(short)-FL-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMy-CTCF-GFP

   Plasmid

   #50574

   Purpose

   Retroviral vector endoding for mouse CTCF fused to GFP

   Depositor

   Rafael Casellas

   Article

   Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

   Insert

   Ampicilin
   

   Use

   Retroviral

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR- flag-Lag16-fibcon-iCAM1

   Plasmid

   #205212

   Purpose

   This vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUMO-YHRC

   Plasmid

   #54336

   Purpose

   Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

   Depositor

   Claes Andréasson

   Article

   Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

   Type

   Empty backbone

   Tags

   6His-SUMO (yeast gene: SMT3)

   Expression

   Bacterial

   Promoter

   T7-LacO

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLTR-X-PURO

   Plasmid

   #58246

   Purpose

   GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   Puro(R)
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   TetR-NLS-T2A

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-CMVSP6-NR2F2-SV40-PURO

    Plasmid

    #138361

    Purpose

    Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2, with puromycin driven by SV40 for selection
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEE491

    Plasmid

    #149292

    Purpose

    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Promoter

    TDH3 and TEF1

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR- flag-Lag6-iCAM-1

    Plasmid

    #205210

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag6 anti-GFP nanobody
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR- flag-Lag18-iCAM-1

    Plasmid

    #205207

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag18 anti-GFP nanobody
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR- flag-Lag42-iCAM-1

    Plasmid

    #205205

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag42 anti-GFP nanobody
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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