We narrowed to 5,405 results for: dre

1. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

   Plasmid

   #159575

   Purpose

   Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

   Plasmid

   #159576

   Purpose

   Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT/TO/HIS/FLAG/HA-HNRNPD

   Plasmid

   #38066

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   HNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

   Plasmid

   #218972

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV LTR

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIC-ZB-SPRTNwt-FL

   Plasmid

   #110217

   Purpose

   Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00035 (aka pMG0784)

   Plasmid

   #210768

   Purpose

   Lentiviral vector to express human ADRM1

   Depositor

   Raj Chari

   Article

   Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

   Insert

   ADRM1 (ADRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Hygro-EF1A-EGFP-PARP1

   Plasmid

   #176146

   Purpose

   EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVtrunc Affimer6-msfGFPΔN12

   Plasmid

   #231550

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. sFlt1-i13 V5

    Plasmid

    #86044

    Purpose

    human Soluble Flt1 isoform i13 (terminates in intron 13)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform i13 (FLT1 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform i13 (terminates in intron 13)

    Promoter

    CMV

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-nuc-GsCT

    Plasmid

    #205517

    Purpose

    Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-GsCT (GNAS Synthetic, Human)
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid

    #232720

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    ABE8e-dCas9(NRCH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within Cas9 D10A H840A

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-HA-UBE3BΔHECT-IRES-Puro

    Plasmid

    #107347

    Purpose

    Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3BΔHECT (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion HECT domain (757-end)

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-enCas12a-2xNLS

    Plasmid

    #209021

    Purpose

    Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    enCas12a-2xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. sFlt1-e15a V5

    Plasmid

    #86045

    Purpose

    human Soluble Flt1 isoform e15a (terminates in exon 15a)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform e15a (FLT1 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform e15a (terminates in exon 15a)

    Promoter

    CMV

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EF-1-alpha

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMVtrunc Affimer6-sfCherry2ΔN12

    Plasmid

    #231551

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Use

    Tags

    sfCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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