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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 2621 - 2640 of 11938 results

  1. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Publication
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad family member 4 (SMAD4 Human, Mouse)
    Use
    Luciferase
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49383-plasmid-map-sequence-id-73406

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  2. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  5. pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

    Plasmid
    #118000
    Purpose
    Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.
    Depositor
    Mathias Pribil
    Publication
    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102
    Insert
    Derlin1-mOrange2
    Use
    Plant/bacteria binary vector
    Expression
    Plant
    Promoter
    Ubiquitin-10
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    118000-plasmid-map-sequence-id-229409

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  6. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Publication
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  8. NSUN5

    Plasmid
    #25491
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NSUN5 (NSUN5 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. myc-BioID-Rab7

    Plasmid
    #121049
    Purpose
    Expresses myc tagged BioID and human Rab7a in mammalian cells
    Depositor
    Gia Voeltz
    Publication
    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    BioID
    Rab7A
    Tags
    myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121049-plasmid-map-sequence-id-235292

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  16. EGFP-GRAM-H

    Plasmid
    #211700
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells
    Depositor
    Yasunori Saheki
    Publication
    Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211700-plasmid-map-sequence-id-419222

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  17. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid
    #178057
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.
    Depositor
    Edward Boyden
    Publication
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-FLAG
    Use
    AAV
    Tags
    FLAG-MBP
    Expression
    Mammalian
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178057-plasmid-map-sequence-id-349209

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Showing: 2621 - 2640 of 11938 results