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We narrowed to 6,963 results for: pcDNA3.1+

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Showing: 2621 - 2640 of 6963 results

  2. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

    Plasmid
    #202606
    Purpose
    Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)
    Depositor
    Francois St-Pierre
    Publication
    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JEDI-1P-P2A-mCherry-CAAX
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    202606-plasmid-map-sequence-id-397595

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  3. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid
    #168099
    Purpose
    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Publication
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nUbc-OGA(544-706)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    168099-plasmid-map-sequence-id-336059

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  4. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    49061-plasmid-map-sequence-id-72183

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  5. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid
    #140893
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
    Depositor
    Yasushi Okamura
    Publication
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…
    Available since
    May 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    140893-plasmid-map-sequence-id-297104

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  6. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    16214-plasmid-map-sequence-id-248256

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  9. pcDNA3.1-Gαi1-Gβ1-Gγ2-GFP2

    Plasmid
    #254719
    Purpose
    Expresses G protein heterotrimer with Gαi1, Gβ1 and Gγ2-GFP2 fusion. Vector used for G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048).
    Depositor
    Cornelius Gati
    Publication
    Khan et al Nature. 2025 Dec;648(8094):755-763. doi:
    Inserts
    Gαi1 (GNAI1 Human)
    Gβ1 (GNB1 Human)
    Gγ2 (GNG2 Human)
    Tags
    GFP2
    Expression
    Mammalian
    Promoter
    CMV, IRES, and T2A
    Available since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254719-plasmid-map-sequence-id-509988

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  13. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , T1785P)-HA

    Plasmid
    #239773
    Purpose
    Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C, T1785P
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239773-plasmid-map-sequence-id-477101

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  15. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Publication
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  18. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid
    #216309
    Purpose
    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216309-plasmid-map-sequence-id-433372

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  19. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid
    #208172
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)
    Depositor
    Syed Saif Hasan
    Publication
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208172-plasmid-map-sequence-id-432648

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  20. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid
    #208173
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Publication
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208173-plasmid-map-sequence-id-436198

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Showing: 2621 - 2640 of 6963 results