We narrowed to 16,048 results for: sgRNA
-
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA EMX1
Plasmid#128121PurposeCo-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA EMX1 + GFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRSV/U6Available SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP3
Plasmid#75165PurposeLentiCRISPR-EGFP with sgRNA targeting human RIP3DepositorInsertRIP3 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#227773PurposeExpression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRPromoterU6 and tp1Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#226834PurposeExpression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRPromoterOlactb and U6Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3
Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 sgRipk4 v3
Plasmid#158048Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9DepositorInsertRipk4 sgRNA
ExpressionMammalianPromoterU6Available SinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2
Plasmid#117406PurposeSpyCas9 sgRNA 2 targeting TLR2.0DepositorInsertSpyCas9 sgRNA targeting TLR 2.0
UseLentiviralExpressionMammalianPromoterU6Available SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAG4E
Plasmid#176868PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_4E
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG5
Plasmid#176869PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_5
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG6E
Plasmid#176870PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_6E
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG2
Plasmid#176864PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_2
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG3
Plasmid#176866PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_3
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG4
Plasmid#176867PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_4
UseCRISPRMutationWTAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB301TOR-CRISPR
Plasmid#164797PurposeFor gene editing in oomycetesDepositorInsertsPsNLS-hSpCas9
sgRNA expression cassette
UseOomycete expressionPromoterHAM34 and RPL41Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBEclos-cbei2385-g2-opt
Plasmid#118215PurposeCRISPR-mediated base editing in ClostridiumDepositorInsertApobec1, Casp nickase, UG1-sgRNA
ExpressionBacterialPromoterPtmAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#113398Purposeexpresses gRNA for Cas9 FRT targettingDepositorInsertexo, beta, gam, sgRNA-FRT
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only