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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,910 results for: LIC

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Showing: 2661 - 2680 of 8910 results

  2. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  4. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  10. pFRLuc-CoV2-FLucStop

    Plasmid
    #177617
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).
    Depositor
    Junjie Guo
    Publication
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    In-frame stop codon added between FLuc and FSE
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177617-plasmid-map-sequence-id-350043

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  14. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  17. p2,0_3M5M_eGFP

    Plasmid
    #135474
    Purpose
    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter
    Depositor
    Elke Mühlberger
    Publication
    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Marburg virus minigenome, eGFP reporter
    Expression
    Mammalian
    Mutation
    None
    Promoter
    T7
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135474-plasmid-map-sequence-id-268921

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  18. p2,0_3M5M_luciferase

    Plasmid
    #135475
    Purpose
    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter
    Depositor
    Elke Mühlberger
    Publication
    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Marburg virus minigenome, luciferase reporter
    Expression
    Mammalian
    Mutation
    None
    Promoter
    T7
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    135475-plasmid-map-sequence-id-301586

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Showing: 2661 - 2680 of 8910 results