We narrowed to 9,098 results for: Pol;

1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

   Plasmid

   #129044

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA4 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

   Plasmid

   #129045

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA5 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

   Plasmid

   #129046

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA6 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-Flag-BRD4-WT

   Plasmid

   #90331

   Purpose

   expresses Wild type BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA5-Flag-BRD4-BD

   Plasmid

   #90005

   Purpose

   expresses BD mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 140N to 140A, 433N to 433A

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Neo Myr Flag RPS6KB1

   Plasmid

   #20624

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   RPS6KB1 (RPS6KB1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pWZL Neo Myr Flag RPS6KB2

   Plasmid

   #20625

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   RPS6KB2 (RPS6KB2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pWZL Neo Myr Flag IKBKE

   Plasmid

   #20504

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Human Genome-Wide Reduced Double-gRNA library

   Pooled Library

   #137999

   Purpose

   This gRNA library targets the human coding genome, aimed to reduce the scale of genome-wide perturbation screens up to fivefold compared to conventional methods, without sacrificing performance.

   Depositor

   Leopold Parts

   Article

   Peets et al bioRxiv 859652

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5-Flag-BRD4-7D

    Plasmid

    #90007

    Purpose

    expresses 7D mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484D, 488S to 488D, 492S to 492D,…

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-Flag-BRD4-7A

    Plasmid

    #90006

    Purpose

    expresses 7A mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484A, 488S to 488A, 492S to 492A,…

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid

    #52093

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid

    #52092

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGLU_82

    Plasmid

    #225783

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[abr]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGLU_100

    Plasmid

    #225813

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[Himar]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGLU_118

    Plasmid

    #225798

    Purpose

    RS421 library variant 13550

    Depositor

    Nili Ostrov

    Insert

    13550_[Tn5]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLfa-PUb-MCS

    Plasmid

    #52908

    Purpose

    Aedes aegypti polyubiquitin promoter followed by MCS

    Depositor

    Zach Adelman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae aegptyi polyubiquitin

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-T4gene43

    Plasmid

    #165524

    Purpose

    Polymerase that replicates viral genomic DNA.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    T4 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge