We narrowed to 7,485 results for: addgene/1000

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CMV:AsCpf1-2A-GFP-U6-INS-sg

Plasmid

#194719

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (INS Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A DD 590-702

Plasmid

#193927

Purpose

Mammalian expression of GFP-tagged Kif 3A DD 590-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3A DD 590-702

Plasmid

#193928

Purpose

Mammalian expression of Halo-tagged Kif 3A DD 590-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A CC 419-589

Plasmid

#193925

Purpose

Mammalian expression of GFP-tagged Kif 3A CC 419-589

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)

Plasmid

#190746

Purpose

Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

CD86 (CD86 Human)

Tags

mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_FLT4

Plasmid

#187769

Purpose

MAC-tagged gene expression of human FLT4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FLT4 (FLT4 Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_KLF5

Plasmid

#187747

Purpose

MAC-tagged gene expression of human KLF5

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

KLF5 (KLF5 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NK72

Plasmid

#176608

Purpose

To test macrophage engulfment initiation. Lacks the FcyR chain.

Depositor

Meghan Morrissey

Article

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

CD86 (CD86 Human)

Use

Lentiviral

Tags

HA, SNAP-tag and mEGFP

Expression

Mammalian

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj16

Plasmid

#173146

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj16a (kcnj16 Zebrafish)

Use

Pcr cloning vector

Mutation

Stop codon removed

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj19b

Plasmid

#173149

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj19b (kcnj19b Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-16a-dendra2

Plasmid

#105434

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-16a Dendra2 (mir16a Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-26b-dendra2

Plasmid

#105436

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-26b Dendra2 (mir26b Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-27b-dendra2

Plasmid

#105437

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-27b Dendra2 (mir27b Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-152-dendra2

Plasmid

#105440

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-152 Dendra (mir152 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-181c-dendra2

Plasmid

#105441

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-181c Dendra2 (mir181c Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-182-dendra2

Plasmid

#105442

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-182 Dendra2 (mir182 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-454a-dendra2

Plasmid

#105444

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-454a Dendra2 (mir454a Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-734-dendra2

Plasmid

#105445

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-734 Dendra2 (mir734 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-10c-dendra2

Plasmid

#105433

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-10c Dendra2 (mir10c Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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