We narrowed to 10,670 results for: nar;
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Plasmid#211781PurposeAviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-EZH2
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUDC175
Plasmid#103019PurposeS. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoterDepositorInsertFncpf1
UseTags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA-Ago2-Y529E
Plasmid#215842PurposeExpression of AGO2-Y529E mutantDepositorInsertHuman AGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationAgo2-Y529EPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEA1
Plasmid#176527PurposeDelivers all prime editing nickase components in a single plasmidDepositorInsertCbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseCRISPRTagsExpressionMammalianMutationPromoterCbH for Cas9, hU6 for gRNAsAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP787_SnoopCatcher-EZH2-FL-CO
Plasmid#211780PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18
Plasmid#68827PurposeExpresses c-myc-tagged human RAD18DepositorInsertc-myc tagged human RAD18 (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ScFv-2ERT2-VPH
Plasmid#120556PurposeEncode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1DepositorInsertscFvGCN4, GB1, ERT2, VP64, P65 and HSF1
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2
Plasmid#206261PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.DepositorTypeEmpty backboneUseMultimate/gateway entr 2TagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-luciferase
Plasmid#113354Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)
Plasmid#120297PurposeAAV vector for expression of AcrIIA4 (no miR binding sites, control vector)DepositorInsertAcrIIA4
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c
Plasmid#137884PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]DepositorInsertAtTAS1c-D2-B/c
UseTagsExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …Promoter2x35SAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP16 (B1014)
Plasmid#92036PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.DepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 (intact NLS) with VP16 activ…ExpressionMammalianMutationPromoterT7Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-NFIB-T2A-miRFP670
Plasmid#187222PurposeExpresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertNuclear Factor I B (NFIB Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only