We narrowed to 8,876 results for: sgRNA

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pYPQ141-ZmUbi-RZ-Aa3.8.4

Plasmid

#136375

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC39

Plasmid

#62333

Purpose

sgRNA + 1x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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PRPF4B gRNA (BRDN0001146164)

Plasmid

#76428

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPF4B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPF4B

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ZAK gRNA (BRDN0001145178)

Plasmid

#75596

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ZAK gRNA (BRDN0001146540)

Plasmid

#75597

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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B52 + PIK3R1 sgSTOP

Plasmid

#100714

Purpose

B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PIK3R1 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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ADCK3 gRNA (BRDN0001148864)

Plasmid

#77322

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146785)

Plasmid

#77412

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001148893)

Plasmid

#77413

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146903)

Plasmid

#77414

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #5

Plasmid

#247545

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #4

Plasmid

#247544

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #3

Plasmid

#247543

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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cBEST2

Plasmid

#234658

Purpose

Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

Depositor

Mingzi M. Zhang

Article

Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

Inserts

spCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

kasO*17tss and kasOp*

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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