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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,802 results for: pcr

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Showing: 2681 - 2700 of 4802 results

  1. GGE038

    Plasmid
    #120785
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120785-plasmid-map-sequence-id-263183

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  2. GGE067

    Plasmid
    #120784
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120784-plasmid-map-sequence-id-263182

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  5. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  6. GGE368

    Plasmid
    #120737
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_Nat)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_Nat
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120737-plasmid-map-sequence-id-259151

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  7. pFRT-Tet 129

    Plasmid
    #33359
    Depositor
    Doug Mortlock
    Insert
    TetR cassette
    Use
    Mouse Targeting
    Tags
    FRT site
    Available Since
    Jan. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. GGE146

    Plasmid
    #120742
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P1_4UASxpr-TEF
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    120742-plasmid-map-sequence-id-331625

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  11. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

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  16. SSTR2-DuET

    Plasmid
    #213377
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR2 (SSTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213377-plasmid-map-sequence-id-430733

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  19. CNR2-DuET

    Plasmid
    #213214
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CNR2 (CNR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213214-plasmid-map-sequence-id-430776

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Showing: 2681 - 2700 of 4802 results