We narrowed to 8,091 results for: sms

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Publication

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

Plasmid

#86016

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Publication

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine

Promoter

CMV IE

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

Plasmid

#221551

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Publication

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGI3EM17

Plasmid

#210569

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Publication

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pM9-BetaGal-8C

Plasmid

#172489

Purpose

Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

Depositor

Siegfried Musser

Publication

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

M9-BetaGal-8C

Tags

6xHis and M9 nuclear localization sequence (from …

Expression

Bacterial

Mutation

BetaGal has two cysteine mutations (C77A, C1022S)…

Promoter

T7

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Publication

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Publication

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-POP5-C1

Plasmid

#134543

Purpose

Expresses YFP-POP5 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

POP5 (POP5 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp14-C1

Plasmid

#134540

Purpose

Expresses YFP-Rpp14 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp14 (RPP14 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTW194b63

Plasmid

#136930

Purpose

Directed evolution of SpCas10

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

npuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Mutation

none

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Fibrillarin-C1

Plasmid

#134538

Purpose

Expresses YFP-Fibrillarin fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fibrillarin (FBL Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4-miR30-TRIM5

Plasmid

#132932

Purpose

Lentivector for TRIM5 knockdown and puromycin resistance

Depositor

Jeremy Luban

Publication

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

TRIM5 shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS rev

Plasmid

#101327

Purpose

Encodes codon optimized NL4-3 rev

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 rev

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Publication

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1-GFP

Plasmid

#108495

Purpose

expression of vsv-CenpB-1-166-Sgo1-EGFP

Depositor

Susanne Lens

Publication

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Sgo1 N61I

Plasmid

#108496

Purpose

expression of vsv-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Publication

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Publication

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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