We narrowed to 3,467 results for: ttl

1. pDGE498

   Plasmid

   #153271

   Purpose

   M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV306-ribo-Sec-APEX2

   Plasmid

   #111701

   Purpose

   Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Use

   Tags

   V5 and mpt63 signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60-ribo

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC111

   Plasmid

   #118906

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
   

   Use

   Tags

   LPETGg, HIS6

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-SERTN177E-myc

   Plasmid

   #107474

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R596Q

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-SERTY289I-myc

   Plasmid

   #107480

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N177E

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-SERTK37Q-myc

   Plasmid

   #107476

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K37Q

   Promoter

   CMV

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-SERTL406I-myc

   Plasmid

   #107487

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L406I

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-SERTE392M-myc

   Plasmid

   #107486

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E392M

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-SERTT233F-myc

   Plasmid

   #107481

   Purpose

   Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activity

   Depositor

   Erik Procko

   Article

   Young et al bioRxiv 2021.04.19.440442

   Insert

   Serotonin Transporter (SERT) (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T233F

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-SERTI576V-myc

    Plasmid

    #107473

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I576V

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-SERTL550V-myc

    Plasmid

    #107472

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transport

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L550V

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR221-CMV.Pro

    Plasmid

    #79486

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CMV Promoter
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive (derepressed P-lac)

    Available since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE497

    Plasmid

    #153249

    Purpose

    M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge