We narrowed to 4,214 results for: Bre;

1. pLenti-VQRRA-PGK-Puro

   Plasmid

   #110858

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-Fascin-Promoter (-402-0)

   Plasmid

   #89823

   Purpose

   Promoter luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FRT2-H2B-EGFP-V5-2A (IG157)

   Plasmid

   #99623

   Purpose

   To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   H2B-EGFP-V5
   

   Use

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR_BRD003-sgRBFOX1-2098

   Plasmid

   #115452

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgRBFOX1-2098 (RBFOX1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXPR_BRD003-sgRBFOX1-2083

   Plasmid

   #115451

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgRBFOX1-2083 (RBFOX1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETDuet-msGC-NT21

   Plasmid

   #78102

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 2…

   Promoter

   T7

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETDuet-msGC-NT13

   Plasmid

   #75087

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 4…

   Promoter

   Available since

   May 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AcrIIC1X* (AcrIIC1X-mCherry chimera)

   Plasmid

   #128114

   Purpose

   Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

   Depositor

   Dominik Niopek

   Article

   Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

   Insert

   AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-VRERRA-PGK-Puro

    Plasmid

    #110859

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

    Plasmid

    #112842

    Purpose

    transient/stable overexpression of the central domain of Catulin.

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Catulin-alpha-1 (Ctnnal1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1 domain of Catulin

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Synaptotagmin-10 [N269/73R]

    Plasmid

    #114558

    Purpose

    Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-VQR-P2A-Puro

    Plasmid

    #110848

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLIK-Neo-TmiR-Gb2

    Plasmid

    #25744

    Purpose

    Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Gb2 (Gnb2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FRT3-Mb2-HA-mTfp1-2A (IG155)

    Plasmid

    #99621

    Purpose

    To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2-HA-mTfp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FRT1-Mb2YFP-2A (IG153)

    Plasmid

    #99619

    Purpose

    To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2EYFP
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FRT2-Mb2Tomato-2A (IG154)

    Plasmid

    #99620

    Purpose

    To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FRT3-HA-H2B-Cerulean-2A (IG158))

    Plasmid

    #99624

    Purpose

    To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-VRERRA-P2A-Puro

    Plasmid

    #110852

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIS1-mutant RhoA 3'UTR

    Plasmid

    #26090

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    mutant RhoA 3'UTR (RHOA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Both miR-31 binding sites in 3’ UTR mutagenized. …

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only