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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,026 results for: Cas9

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Showing: 2701 - 2720 of 22026 results

  1. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  5. pJHG003

    Plasmid
    #126454
    Purpose
    Expresses Cas9-XTEN-dTdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
    Expression
    Mammalian
    Mutation
    Added D343E and D345E to inactivate TdT.
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126454-plasmid-map-sequence-id-245973

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  6. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

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  8. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104406-plasmid-map-sequence-id-204465

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  9. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Publication
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118632-plasmid-map-sequence-id-230217

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  10. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  11. pX330A_D10A-1x5

    Plasmid
    #58775
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58775-plasmid-map-sequence-id-91085

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  15. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  16. pX330A_D10A-1x5

    Plasmid
    #58775
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58775-plasmid-map-sequence-id-91085

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  17. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50593-plasmid-map-sequence-id-451910

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  20. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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Showing: 2701 - 2720 of 22026 results