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pEF-1a_RfxCas13d-HA-IRES-BFP-2A-BSD

Plasmid

#251220

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven RfxCas13d expressing cells

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD

Plasmid

#251219

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LbuCas13a

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE

Plasmid

#251210

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

dEGFP-Y66S

Use

Lentiviral

Expression

Mammalian

Mutation

Y66S

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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nSpG UGI Puro

Plasmid

#235046

Purpose

Control nickase Cas9

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJL178

Plasmid

#243687

Purpose

Stably expresses fusion of SUPV3L1(1-114aa) and ALDH18A1(106-795aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1-(1-114aa)-ALDH18A1(106-795aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-TdTomato-WPRE-polyA

Plasmid

#241828

Purpose

AAV construct for constitutive expression of TdTomato.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

TdTomato-WPRE-polyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12-Clover

Plasmid

#215527

Purpose

Expression vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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EFS-mRuby2-bGH

Plasmid

#235253

Purpose

ComMAND base gene regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

Plasmid

#207172

Purpose

expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD GalO linker

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DpbCas12e

Plasmid

#188495

Purpose

Expresses FLAG tagged DpbCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PlmCas12e

Plasmid

#188496

Purpose

Expresses FLAG tagged PlmCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12j3

Plasmid

#188497

Purpose

Expresses FLAG tagged Cas12j3

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-LAP1-mCherry

Plasmid

#159525

Purpose

Expresses mCherry from LAP1 promoter

Depositor

Esteban Engel

Article

Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

LAP1

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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