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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 2701 - 2720 of 16276 results

  2. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Publication
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  3. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105003-plasmid-map-sequence-id-202164

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  11. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Publication
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Expression
    Bacterial
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  12. pJFA334E9

    Plasmid
    #49237
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    49237-plasmid-map-sequence-id-72803

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  19. iOn-CAG∞MCS

    Plasmid
    #154013
    Purpose
    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
    Depositor
    Jean Livet
    Publication
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    MCS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154013-plasmid-map-sequence-id-307168

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Showing: 2701 - 2720 of 16276 results