We narrowed to 22,423 results for: streptococcus -(crispr) -(cas9)

Showing: 2701 - 2720 of 22423 results

Results per page:

Sort results by:

pG3GB411

Plasmid

#134748

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

3XFLAG-Cas9

Expression

Mammalian

Promoter

CBh; U6

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

3XFLAG-Cas9

Expression

Mammalian

Promoter

CBh; U6

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dMET

Plasmid

#176031

Purpose

A knock-out vector for dog MET

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dMET

Plasmid

#176031

Purpose

A knock-out vector for dog MET

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-PE SpRY

Plasmid

#179082

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-PE SpRY

Plasmid

#179082

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5h

Plasmid

#160295

Purpose

Yeast CRISPR plasmid targeting the hphMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5n

Plasmid

#160296

Purpose

Yeast CRISPR plasmid targeting the natMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5k

Plasmid

#160294

Purpose

Yeast CRISPR plasmid targeting the kanMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5h

Plasmid

#160295

Purpose

Yeast CRISPR plasmid targeting the hphMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5n

Plasmid

#160296

Purpose

Yeast CRISPR plasmid targeting the natMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY5k

Plasmid

#160294

Purpose

Yeast CRISPR plasmid targeting the kanMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge