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pPB-CAG-mNanogHD-pgk-hph

Plasmid

#74909

Purpose

Mammalian expression of mNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK360

Plasmid

#71571

Purpose

Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

acetyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Mutation

changes threonine-2 to alanine

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJK385

Plasmid

#71572

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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CaRQ

Plasmid

#34558

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

CaRQ (RHOA Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
MET FLAG72QDProCFPp303

Plasmid

#15590

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
SNX26-PX (56-172)

Plasmid

#119105

Purpose

Bacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX26-PX (56-172)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX28-PX (1-124)

Plasmid

#119107

Purpose

Bacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX28-PX (1-124)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX34-PX (1-130)

Plasmid

#119113

Purpose

Bacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX34-PX (1-130)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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IRAS-PX (13-124)

Plasmid

#119118

Purpose

Bacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

IRAS-PX (13-124)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-dTomato-2xrGBD

Plasmid

#222638

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-dTomato-2xrGBD

Use

Lentiviral

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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CAG_IGF1R_EGFP

Plasmid

#199628

Purpose

FRET donor for IGF1R sensor

Depositor

Ryohei Yasuda

Article

Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

Insert

IGF1R (IGF1R Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6_AF

Plasmid

#148738

Purpose

Bacterial Expression of HsNot6

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot6 (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_TBL_bridge_RNA_polIII_term

Plasmid

#251496

Purpose

Expression of the Target Binding Loop (TBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Target Binding Loop (TBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term

Plasmid

#251497

Purpose

Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Donor Binding Loop (DBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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TV_ANXA1-p2a-mScarlet-nls-p2a-icasp9_pgk-blast

Plasmid

#251171

Purpose

Targeting vector for C-terminal knockin at ANXA1 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9.

Depositor

Hugo Snippert

Insert

p2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human ANXA1 (ANXA1 Human)

Use

CRISPR

Tags

p2a-mScarlet-nls-p2a-icasp9

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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