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Plasmid#71352Purposecsd6, construct:13-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_1-330/pET28b(+) N-tag
Plasmid#71350Purposecsd6, construct:1-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SL542
Plasmid#49951PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-8 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
SL544
Plasmid#49970PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-10 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL545
Plasmid#49953PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-11 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL543
Plasmid#49952PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-9 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL541
Plasmid#49950PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-7 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL540
Plasmid#49949PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-6 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4-no linker
Plasmid#176925Purposeexpress Granulin 4 without linker 5 in mammalian cellsDepositorInsertGranulin 4 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A-2A-GFP
Plasmid#118270PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-Ngn3PDX1MafA
Plasmid#114303PurposeConstitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptidesDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralTagsExpressionMutationNgn3, PDX1 and MafA proteins are expressed from a…PromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HSPA9_HSP70
Plasmid#109776PurposeProtein expression and purification of HSPA9_HSP70DepositorInsertHSPA9_HSP70 (HSPA9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GLP1R-DuET
Plasmid#213253PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGLP1R (GLP1R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-BRCA2
Plasmid#99395PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2DepositorInsertBRCA2 (BRCA2 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A1_GTP-EFTU
Plasmid#109861PurposeProtein expression and purification of EEF1A1_GTP-EFTUDepositorInsertEEF1A1_GTP-EFTU (EEF1A1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_UCN1.0
Plasmid#208669PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent mannerDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-TfR
Plasmid#12130DepositorInserttransferrin receptor (TFRC Human)
UseTags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only