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PB-2G-CNCB

Plasmid

#236764

Purpose

A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter, human U6 promoter, CAG promoter

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196990

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

VEGFR2

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m1

Plasmid

#204463

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR A127N mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m2

Plasmid

#204464

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L128C mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFP

Plasmid

#232610

Purpose

Pan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

LITE-1 (lite-1 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

Plasmid

#232608

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::prdx-2G::SL2::his-24::tagRFP

Plasmid

#232609

Purpose

Pan-neuronal expression of 'PRDX-2' using a genomic fragment and fluorophore 'tagRFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

PRDX-2 (prdx-2 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0140 CMVTO-Renin-ECL-CD4-tevs-RXR6.2

Plasmid

#231199

Purpose

ER-retained renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin-ECL-CD4-tevs-RXR6.2 (REN Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0151 CMVTO-Renin(H2M)-ECL

Plasmid

#231201

Purpose

Membrane-bound human-to-mouse (H2M) renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL (REN Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CMVTO-hIL2 (AV)

Plasmid

#231226

Purpose

hIL2

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2 (IL2 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES2_hMela(CT+IL3 mGluR6)

Plasmid

#191343

Purpose

Optogenetic tool

Depositor

Sonja Kleinlogel

Article

Kralik et al Commun Biol. 2022 Oct 20;5(1):1116. doi:

Insert

hMela(CT+IL3 mGluR6)

Tags

IRES_Turbo

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2_hMela(CT mGluR6)

Plasmid

#191344

Purpose

Optogenetic tool

Depositor

Sonja Kleinlogel

Article

Kralik et al Commun Biol. 2022 Oct 20;5(1):1116. doi:

Insert

hMela(CTmGluR6)

Tags

IRES_Turbo

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p8270 LentiCRISPR v2 hygro sgSIGIRR-3

Plasmid

#193979

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV:: NES-FKBP-iLID-LRP6c(△1-64)

Plasmid

#221775

Purpose

Expresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: NES-FKBP-iLID-LRP6c

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_CMV_Prss-Halo-KDEL

Plasmid

#216256

Purpose

CMV promoter-regulated ER targeted HaloTag expression vector

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

ER retained HaloTag

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Plasmid

#221776

Purpose

Expresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB

Plasmid

#219557

Purpose

PM-targeted iLID-LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-iLID-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF2 CTF-FLAG

Plasmid

#217695

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF2 (ADGRF2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-429

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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