We narrowed to 24,008 results for: CHI

1. mBFP-APP-mGFP

   Plasmid

   #196694

   Purpose

   Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mEGFP and mtagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

   Plasmid

   #128327

   Purpose

   Reporter to evaluate YAP1/TEAD-mediated gene transcription

   Depositor

   Yutaka Hata

   Article

   Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

   Inserts

   H2B mCherry
   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-APP-mGFP

   Plasmid

   #196704

   Purpose

   Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mCherry and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-APPP1-mGFP

   Plasmid

   #196705

   Purpose

   As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mCherry and mEGFP

   Expression

   Mammalian

   Mutation

   Lys to Val substitution at position 612

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV-EF1a-mCherryBCL11B-W

   Plasmid

   #208758

   Purpose

   Lentiviral vector expressing mCherry fused with BCL11B cDNA

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   BCL11B (BCL11B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(SMARCA4(15))-PGKpuro2ABFP-W

   Plasmid

   #200462

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(15) (SMARCA4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-W

   Plasmid

   #200464

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43) (SMARCA4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-IDO2-p2a-eGFP

   Plasmid

   #187027

   Purpose

   Expresses IDO2 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 2 (IDO2 Human)
   

   Use

   Tags

   P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

   Plasmid

   #216155

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic splice site and C-terminal FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mBFP-APPP1-mGFP

    Plasmid

    #196695

    Purpose

    As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

    Plasmid

    #211968

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

    Plasmid

    #211966

    Purpose

    Express gRNA against JARID2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting JARID2 (JARID2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid

    #153062

    Purpose

    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutagenesis to insert a stop codon at aa 298 in B…

    Promoter

    EF1α

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AP-APP

    Plasmid

    #196706

    Purpose

    Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    AP, Avitag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN879

    Plasmid

    #190693

    Purpose

    Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

    Depositor

    Shinsuke Niwa

    Article

    Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

    Insert

    mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

    Plasmid

    #211986

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge