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We narrowed to 7,486 results for: CIT

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Showing: 2741 - 2760 of 7486 results

  3. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  6. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid
    #165430
    Purpose
    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    FHS-paCaMKII(SD)
    Use
    AAV
    Tags
    FHS(Flag, HisX6, Strep) tag
    Expression
    Mammalian
    Mutation
    C450A, L514K, G528A, L531E, N538E: Light-insensit…
    Promoter
    CaMKII0.4
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165430-plasmid-map-sequence-id-325945

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  11. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  19. pAAV-shCTRL

    Plasmid
    #181875
    Purpose
    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    control shRNA with no known targets in the mouse genome
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181875-plasmid-map-sequence-id-355955

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  20. CMV-GEX-GECO1

    Plasmid
    #32443
    Purpose
    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEX-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 2741 - 2760 of 7486 results