We narrowed to 16,247 results for: GRN

1. pX458_mCherry-TCOF1-gRNA

   Plasmid

   #237633

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TCOF1 gRNA (TCOF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458_mCherry-FUS-gRNA

   Plasmid

   #237685

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to FUS locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   FUS gRNA (FUS Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pk335-NT-1mer-gRNA

   Plasmid

   #227500

   Purpose

   non-targeting control guide

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNF168 C-terminal sgRNA

   Plasmid

   #207100

   Purpose

   pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GAGATGCACAAAGTAAGGCC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. RIF C-terminal sgRNA

   Plasmid

   #207096

   Purpose

   pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AATACTAAATAGAATTTTCA
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. REV7 C-terminal sgRNA

   Plasmid

   #207095

   Purpose

   pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GCTCATAAAGGCAGCTGAGG
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Halo XLF sgRNA

   Plasmid

   #207605

   Purpose

   sgRNA for the insert of the HaloTag at the endogenous loci of XLF

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   GTTCTTCCATctgcaaaaaa
   

   Tags

   None

   Expression

   Mammalian

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-eBFP2-sgRNA_CTCF_ZF1

   Plasmid

   #233090

   Purpose

   Expression vector for a sgRNA against the mouse CTCF ZF1 region and SpCas9-T2A-eBFP2.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   spCas9-T2A-eBFP2 (Ctcf )
   

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. NOLC1 atgRNA pDY2250

   Plasmid

   #219860

   Purpose

   attachment site pegRNA for human NOLC1

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBE-dGFP-gRNA

    Plasmid

    #226862

    Purpose

    Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA giantin/GOLGB1

    Plasmid

    #222315

    Purpose

    CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

    Insert

    gRNA targeting GOLB1 (GOLGB1 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH86966::AtU6p::sgRNA:NbVPE1a/b

    Plasmid

    #223218

    Purpose

    Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbVPE1a/b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Promoter

    Arabidopsis U6 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Promoter

    Arabidopsis U6 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

    Plasmid

    #216170

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

    Plasmid

    #216171

    Purpose

    Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

    Plasmid

    #216172

    Purpose

    Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- Control- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

    Plasmid

    #216173

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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