We narrowed to 3,441 results for: car-t

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TFORF1133

Plasmid

#142734

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CARF (ALS2CR8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A53_STOP

Plasmid

#161209

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A53 (SLC25A53 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)

Viral Prep

#115643-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p><p>Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.</p></p>

Depositor

Samantha Morris

Article

Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

Promoter

Minimal CMV

Tags

EGFP

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only
RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Article

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
rag2:CPSF6_CE_pISceI

Plasmid

#223037

Purpose

Express CPSF6 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CPSF6 (CPSF6 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hcGAS(157-522)

Plasmid

#108676

Purpose

For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

cGAS amino acid 157-522 (CGAS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acid 157-522

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pdest N-FLAG METTL1-WT puro

Plasmid

#223055

Purpose

METTL1-WT gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-WT (METTL1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-CM Neo

Plasmid

#223061

Purpose

METTL1-CM gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

160-163 (LFPD>AFPA)

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:FRS2_CE_pISceI

Plasmid

#223040

Purpose

Express FRS2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

FRS2 (FRS2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

Plasmid

#169168

Purpose

Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6X Histidine Tag, TEV cleavage site, mCherry

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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